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gpr [2019-07-05 11:32:17]
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gpr [2019-07-05 11:32:17]

germination protease (degradation of SASPs)
Locus
BSU25540
Isoelectric point
5.20
Molecular weight
40.12 kDa
Protein length
368 aa Sequence Blast
Gene length
1107 bp Sequence Blast
Function
degradation of SASPs
Product
germination protease (degradation of SASPs)
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,634,505 2,635,611

The protein

Catalyzed reaction/ biological activity

  • degradation of small, acid-soluble spore proteins (SASPs) PubMed
  • Protein family

  • peptidase A25 family (according to Swiss-Prot)
  • Structure

  • 1C8B (from B. megaterium, 68% identity) PubMed
  • Localization

  • inner spore membrane PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigF: sigma factor, PubMed, in SigF regulon
  • SigG: sigma factor, PubMed, in SigG regulon
  • Regulatory mechanism

  • SpoVT: repression, PubMed, in SpoVT regulon
  • Regulation

  • expressed during sporulation in the forespore (SigF, SigG, SpoVT) PubMed
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    Biological materials

    Mutant

  • BKE25540 (gpr::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACAGGGGCCTCCTTCAC, downstream forward: _UP4_TAAACAGTTCTACTTCCTCT
  • BKK25540 (gpr::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACAGGGGCCTCCTTCAC, downstream forward: _UP4_TAAACAGTTCTACTTCCTCT
  • References

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